Parole aux jeunes : apports d’une recherche qualitative au concept de perception de la valeur des études

Conférence présentée lors du colloque « Journée de la recherche sur la motivation au collégial » Acfas, Montréal, 10 mai 201

High resolution thermal and multispectral UAV imagery for precision assessment of apple tree response to water stress

UMR AGAP - équipe AFEF - Architecture et fonctionnement des espèces fruitières(Edited by Pablo Gonzalez-de-Santos and Angela Ribeiro)This manuscript presents a comprehensive methodology to obtain Thermal, Visible and Near Infrared ortho-mosaics, as a previous step for the further image-based assessment of response to water stress of an experimental apple tree orchard. Using this methodology, multi-temporal ortho-mosaics of the field plot were created and accuracy of ortho-rectification and geo-location computed. Unmanned aerial vehicle (UAV) flights were performed on an irrigated apple tree orchard located in Southern France. The 6400 m² plot was composed of 520 apple trees which were disposed in 10 rows. In this field set-up, five well irrigated rows alternated with five rows submitted to progressive summer water constraints. For remote image acquisition, on 4th July, 19th July, 1st August and 6th September UAV flights with three cameras onboard (thermal, visible and near infrared) were performed at solar noon. On 1st August, five successive UAV flights were carried out at 8, 10, 12, 14 and 16 h (solar time). By using selfdeveloped software, frames were automatically extracted from the recorded thermal video and turned in the right image format. The temperature of four different targets (hot, cold, wet and dry bare soil) was continuously measured by the IR120 thermoradiometers during each flight, for radiometric calibration purpose. Based each on thirty images, all ortho-mosaics were successfully obtained. As high spatial resolution imagery requires high precision geo-location, and the root mean squared error (RMSE) of each ortho-mosaic positioning was calculated in order to assess its spatial accuracy. RMSE values were less than twice the pixel size in every case, which allowed a precise overlapping of the mosaics created. Canopy temperature data extracted from thermal images for showed significantly higher temperatures in water stressed trees compared to well irrigated, difference being related to severity of water stress. Thanks to the ultrahigh resolution of remote images obtained (<0.1m spatial resolution for thermal infrared images), and beyond its capacity to delineate efficiently each individual tree, the methodology presented here will also make it possible the analysis of intra-canopy variations and the accurate calculation of vegetation and water stress indices

Acquisition d'images thermiques par drone : corrections radiométriques à partir de données terrain

Thermal images have many applications in agronomy, including crop water stress status assessment. Nowadays, the miniaturization of thermal cameras allows installing them onboard the Unmanned Aerial Vehicles (UAV), but this miniaturization leads to some difficulties: the miniaturized thermal cameras have no temperature control system of their sensor. The instability of the miniaturized camera makes a high drift in the acquisition of temperature data so that acquired thermal images don't fit the real temperature of the studying object, so data have to be continuously corrected. We need to have stable reference on field in order to compute the actual temperature value. In this article we present a method for radiometric correction of UAV remote sensed thermal images. We have implemented a device in order to retrieve ground temperature measurements. This device is composed with four targets (cold, hot, dry soil, wet soil) which measured continuously the target temperature thanks to IR120 (Campbell ®) radio-thermometer. A meteorological station is included in this ground system and acquires air temperature and moisture, solar radiation, wind speed and direction every 10 seconds. The images are radiometrically corrected by linear regression from on ground thermal data collected. Corrected images have been compared with mean canopy surface temperature of a sample of 10 trees measured with radio-thermometers. The results showed a good link between data from on ground radio-thermometer and data from thermal camera after radiometric correction. We can conclude that images obtained by this method are of sufficient quality to be used in vegetation water stress studies. (Résumé d'auteur

Wolbachia Age-Sex-Specific Density in Aedes albopictus: A Host Evolutionary Response to Cytoplasmic Incompatibility?

Wolbachia bacteria have invaded many arthropod species by inducing Cytoplasmic Incompatibility (CI). These symbionts represent fascinating objects of study for evolutionary biologists, but also powerful potential biocontrol agents. Here, we assess the density dynamics of Wolbachia infections in males and females of the mosquito Aedes albopitcus, an important vector of human pathogens, and interpret the results within an evolutionary framework.Wolbachia densities were measured in natural populations and in age controlled mosquitoes using quantitative PCR. We show that the density dynamics of the wAlbA Wolbachia strain infecting Aedes albopictus drastically differ between males and females, with a very rapid decay of infection in males only.Theory predicts that Wolbachia and its hosts should cooperate to improve the transmission of infection to offspring, because only infected eggs are protected from the effects of CI. However, incompatible matings effectively lower the fertility of infected males, so that selection acting on the host genome should tend to reduce the expression of CI in males, for example, by reducing infection density in males before sexual maturation. The rapid decay of one Wolbachia infection in Aedes albopictus males, but not in females, is consistent with this prediction. We suggest that the commonly observed reduction in CI intensity with male age reflects a similar evolutionary process. Our results also highlight the importance of monitoring infection density dynamics in both males and females to assess the efficiency of Wolbachia-based control strategies

Phosphoproteomic analysis identifies supervillin as an ERK3 substrate regulating cytokinesis and cell ploidy

Extracellular signal-regulated kinase 3 (ERK3) is a poorly characterized member of the mitogen-activated protein (MAP) kinase family. Functional analysis of the ERK3 signaling pathway has been hampered by a lack of knowledge about the substrates and downstream effectors of the kinase. Here, we used large-scale quantitative phosphoproteomics and targeted gene silencing to identify direct ERK3 substrates and gain insight into its cellular functions. Detailed validation of one candidate substrate identified the gelsolin/villin family member supervillin (SVIL) as a bona fide ERK3 substrate. We show that ERK3 phosphorylates SVIL on Ser245 to regulate myosin II activation and cytokinesis completion in dividing cells. Depletion of SVIL or ERK3 leads to increased cytokinesis failure and multinucleation, a phenotype rescued by wild type SVIL but not by the non-phosphorylatable S245A mutant. Our results unveil a new function of the atypical MAP kinase ERK3 in cell division and the regulation of cell ploidy

Ultra-deep Spitzer Mid-Infrared Spectroscopy of LIRGs and ULIRGs at z ~ 1-2

We present ultra-deep mid-IR spectra of 48 infrared-luminous galaxies in the GOODS-South field obtained with the InfraRed Spectrograph (IRS) on the Spitzer Space Telescope. These galaxies are selected among faint infrared sources (0.14 - 0.5 mJy at 24 um) in two redshift bins (0.76-1.05 and 1.75-2.4) to sample the major contributors to the cosmic infrared background at the most active epochs. We estimate redshifts for 92% of the sample using PAH and Si absorption features. Only few of these galaxies (5% at z~1 and 12% at z~2) have their total infrared luminosity dominated by emission from AGN. The averaged mid-IR spectra of the z~1 LIRGs and of the z~2 ULIRGs are very similar to the averaged spectrum of local starbursts and HII-like ULIRGs, respectively. We find that 6.2um PAH equivalent widths reach a plateau of ~1 um for L(24 mu) < 1E11 L(sun). At higher luminosities, EW (6.2 mu) anti-correlates with L(24 um). Intriguingly, high-z ULIRGs and SMG lie above the local EW (6.2 um) - L(24 um) relationship suggesting that, at a given luminosity, high-z ULIRGs have AGN contributions to their dust emission lower than those of local counterparts. A quantitative analysis of their morphology shows that most of the luminous IR galaxies have morphologies similar to those of IR-quiet galaxies at the same redshift. All z~2 ULIRGs of our sample are IR-excess BzK galaxies and most of them have L(FIR)/L(1600A) ratios higher than those of starburst galaxies at a given UV slope. The ``IR excess'' (Daddi et al. 2007) is mostly due to strong 7.7 um PAH emission and under-estimation of UV dust extinction. On the basis of the AGN-powered L (6 um) continuum measured directly from the mid-IR spectra, we estimate an average intrinsic X-ray AGN luminosity of L(2-10 keV) = (0.1 +/- 0.6) 1E43 erg/s, a value substantially lower than the prediction by Daddi et al. (2007).Comment: 45 pages, 36 figures, 2 tables, accepted for publication on Ap